L-Phase Variant of Neisseria meningitidis (Albrecht and Ghon) Murray
Strain Designations
55-RS-1-L
Application
Production of L-Phase variant: Parent ---> L-Phase variant ---> revertant (2-5 passages) ---> L-Phase variant (40 passages with penicillin plus 93 passages without penicillin)
Isolation
Derived from existing strain
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Antigenic Properties
Serogroup B
Product Format
freeze-dried
Preceptrol®
no
Type Strain
no
Comments
Relatively stable when grown in the absence of penicillin
Medium
ATCC® Medium 262: Brain heart infusion with agar, yeast extract, sucrose, inactivated horse serum, and penicillin
Growth Conditions
Temperature: 37.0°C
Atmosphere: 5% CO2 plus moisture
Name of Depositor
RG Wittler
Chain of Custody
ATCC <<--RG Wittler<<--R. Roberts
References
Roberts RB, Wittler RG. The L form of Neisseria meningitidis. J. Gen. Microbiol. 44: 139-148, 1966. PubMed: 4960901
Cohen RL, et al. Modified biochemical tests for characterization of L-phase variants of bacteria. Appl. Microbiol. 16: 1655-1662, 1968. PubMed: 4302280
derived from a laboratory culture of Neisseria meningitidis 55-R (ATCC 23247)
