| Comments |
Restriction digests of the clone give the following sizes (kb): HpaI--1.8, 1.2, 0.9 + smaller; HindIII--16.9, 3.6, 3.2, 2.2, 1.9; EcoRI--19.6, 7.5; XbaI--28; AccI--12.3, 7.2, 6.3, 4.4, 1.6. Depositor has verified that our preparation detects the described RFLP. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. RFLPs observed under normal stringency (0.1X SSC). |
| References |
Nakamura Y, et al. New approach for isolation of VNTR markers. Am. J. Hum. Genet. 43: 854-859, 1988. PubMed: 2904220
Yusuke Nakamura, personal communication
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