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Trichomonas vaginalis Donne

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編號:B226114

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產(chǎn)品名稱	Trichomonas vaginalis Donne
商品貨號	B226114
Strain Designations	11769
Application	Sexually Transmitted Disease Research
Biosafety Level	2 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation	Human, Colorado Gen Hosp., Denver, CO, 1958
Product Format	frozen
Storage Conditions	Frozen Cultures: -70°C for 1 week; liquid N₂ vapor for long term storage Freeze-dried Cultures: 2-8°C Live Cultures: See Protocols section for handling information
Type Strain	no
Comments	Requirement for saturated and unsaturated fatty acids Geographic variation In vitro susceptibility to metronidazole
Medium	ATCC^® Medium 2154: LYI Entamoeba medium ATCC^® Medium 361: Modified TYM basal medium (ATCC medium 358) with pH adjusted to 6.0 and 0.2-0.5 ml of heat-inactivated horse serum added per tube before use
Growth Conditions	Temperature: 35°C Atmosphere: Anaerobic Culture System: Axenic; pH 6.0
Cryopreservation	Harvest and Preservation Harvest cells from a culture that is at or near peak density by centrifugation at 800 x g for 5 min. The cells grown in a medium containing agar are concentrated by centrifugation, a solid pellet does not form. The soft pellet is resuspended to desired cell concentration with agar-free supernatant. Adjust the concentration of cells to 2 x 10⁶to 2 x 10⁷/mL in fresh medium. While cells are centrifuging prepare a 10% (v/v) solution of sterile DMSO in fresh medium. Add 1.0 mL of DMSO to an ice cold 20 x 150 mm screw-capped test tube. Place the tube on ice and allow the DMSO to solidify (~5 min) and then add 9.0 mL of ice cold medium. Invert several times to dissolve the DMSO. Allow to warm to room temperature. Mix the cell preparation and the DMSO in equal portions. Thus, the final concentration will be 10⁶ to 10⁷ cells/mL and 5% (v/v) DMSO. The time from the mixing of the cell preparation and DMSO stock solution before the freezing process is begun should no less than 15 min and no longer than 30 min. Dispense in 0.5 mL aliquots into 1.0 mL to 2.0 mL sterile plastic screw-capped cryules (special plastic vials for cryopreservation). Place the vials in a controlled rate freezing unit. From room temperature cool at -1°C/min to -40°C. If the freezing unit can compensate for the heat of fusion, maintain rate at -1°C/min through the heat of fusion. At -40°C plunge into liquid nitrogen. Alternatively, place the vials in a Nalgene 1°C freezing apparatus. Place the apparatus at -80°C for 1.5 to 2 hours and then plunge ampules into liquid nitrogen. (The cooling rate in this apparatus is approximately -1°C/min.) The frozen preparations should be stored in either the vapor or liquid phase of a nitrogen refrigerator. Frozen preparations stored below -130°C are stabile indefinitely. Those stored at temperatures above -130°C are progressively less stabile as the storage temperature is elevated. Vials should not be stored above -55°C. To establish a culture from the frozen state place an ampule in a water bath set at 35°C. Immerse the vial just to a level just above the surface of the frozen material. Do not agitate the vial. Immediately after thawing, do not leave in the water bath, aseptically remove the contents of the ampule and inoculate a 16 x 125 mm screw-capped test tube containing either 9 mL of ATCC medium 361 (completed with serum) or 13 mL ATCC Medium 2154 adjusted to pH 6.0. Incubate the culture at 35°C with the cap screwed on tightly (tube should be vertical for medium 361 or on a 15° horizontal slant for medium 2154).
Name of Depositor	R Samuels
Year of Origin	1958
References	Cornelius DC, et al. Short report: genetic relatedness of Trichomonas vaginalis reference and clinical isolates. Am. J. Trop. Med. Hyg. 83: 1283-1286, 2010. Diamond LS. Axenic cultivation of Trichomonas tenax, the oral flagellate of man. I. Establishment of cultures. J. Protozool. 9: 442-444, 1962. Krieger JN, et al. Geographic variation among isolates of Trichomonas vaginalis: demonstration of antigenic heterogeneity by using monoclonal antibodies and the indirect immunofluorescence technique. J. Infect. Dis. 152: 979-984, 1985. PubMed: 2413147 Samuels R, Stouder DJ. Disc testing of drugs against plated Trichomonas vaginalis. J. Protozool. 9: 249-254, 1962. Shorb MS, Lund PG. Requirement of Trichomonads for unidentified growth factors, saturated and unsaturated fatty acids. J. Protozool. 6: 122-130, 1959.

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