The insert contains the following restriction sites (approximate kb from the 5' end): PstI--1.1; HindIII--1.6; PvuII--0.45. Restriction digests of the clone give the following sizes (kb): EcoRI--2.8, 1.8; BamHI--4.7; HindIII--4.6 + 1 smaller; PstI--4.1, 0.68; SalI--2.8, 1.8. Corresponds to the 5' EcoRI fragment of lambdahPKC-beta115, with the 3' end being the EcoRI site at nt 2027 of the sequence record. Contains most of the coding sequence (nt 65-2086 of GenBank M13975). |
