| 產品名稱 |
pBC102-K22 |
| 商品貨號 |
B226969 |
| Designations |
pBC102-K22 |
| Depositors |
Immunex Corporation, SG Hallquist, Immunex Corporation |
| U.S. Patent |
|
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Biosafety Level |
1 |
| Vector Information |
Size (kb): 9.3999996185302730 Vector: pBC102-K22 (plasmid) Promoters: Promoter ADH2 Construction: pBR322 Marker(s):TRP1,ampR Construct size (kb): 9.399999618530273 Features: marker(s): ampR, TRP1
promoter: ADH2
replicon: pMB1, 2 micron |
| Applications |
YE-type (episomal) shuttle vector expression vector vector permitting construction of fusion proteins vector with sequences for protein export |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--9.4; BamHI--uncut; HindIII--6.5, 2.1, 0.8; PstI--5.8, 1.9, 1.7. Shuttle expression vector utilizing sequences encoding the alpha factor leader peptide to allow secretion of recombinant proteins. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37.0°C |
| References |
Curtis BM, et al. Fusion proteins comprising GM-CSF and IL-3. US Patent 5,073,627 dated Dec 17 1991
Curtis BM, et al. DNA sequences encoding fusion proteins comprising GM-CSF and IL-3. US Patent 5,108,910 dated Apr 28 1992
|
| Shipped |
frozen |
| Shipping Information |
Distributed: freeze-dried |
