| Insert |
DNA: genomic Insert lengths(kb): 40.0 Gene product: corticotropin-releasing factor receptor subtype 2 [CRF2R] Alleles: A6, B1, D2, A5, B2, C2, D1, A1, A2, A2, A1, B1, A1, A2, A3, A4, B2, C1 |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--16, 12, 9, 3.8, 3.5, 3.4, 2.3; HindIII--16, 6.2, 6, 4.4, 4.2, 4, 2.8, 2.2, 1.7; EcoRI--11, 8.2, 8.2, 3.8, 3.6, 3.3, 3.2, 2.25, 2, 1.95; HindIII/EcoRI--10.5, 5.6, 4.2, 3.9; BamHI/HindIII--7.8, 6.0, 4.4, 3.8, 3.2, 3.0, 2.8, 2.3, 2.2, 2.2, 2.0, 1.4. IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture. |
