| 產(chǎn)品名稱(chēng) |
pGEM2MCSF10 [CMCC 2760] |
| 商品貨號(hào) |
B227282 |
| Designations |
pGEM2MCSF10 [CMCC 2760] |
| Species |
Mus musculus, mouse |
| Depositors |
JC Hunter-Cevera |
| Vector |
Construct size (kb): 6.900000095367432 |
| Insert |
DNA: cDNA Insert lengths(kb): 4.0 Gene product: colony stimulating factor, macrophage [Csfm] |
| Insert Size (kb) |
4.0 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
The insert contains the following sites separated by (bp)(approximate): EcoRI- 310- PstI- 630- AvaI- 136- HindIII- 533- BamHI- 1390- HindIII- 932- EcoRI. Restriction digests of the clone give the following sizes (kb): EcoRI--3.85, 2.9; HindIII--3.8, 1.95, 0.95; PstI--3.65, 3.1; BamHI--4.35, 2.35; XbaI--6.8. The insert is oriented in the sense direction relative to the SP6 promoter, such that RNA transcription will give translatable mRNA. |
| Classification |
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod |
| References |
Ladner MB, et al. cDNA cloning and expression of murine macrophage colony-stimulating factor from L929 cells. Proc. Natl. Acad. Sci. USA 85: 6706-6710, 1988. PubMed: 2457916
Jennie C Hunter-Cevera, personal communication
|
