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貨號：B245025
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名稱：Septoria passiflorae Sydow, anamorph

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貨號：B245016
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Primary Dermal Fibroblast Normal; Human, Neonatal (HDFn)

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貨期:

編號:B227285

品牌:Mingzhoubio

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產(chǎn)品名稱 Primary Dermal Fibroblast Normal; Human, Neonatal (HDFn)

商品貨號 B227285

Organism Homo sapiens, human

Tissue Skin

Cell Type Fibroblast

Morphology Spindle-shaped; cells are bipolar and refractile.

Growth Properties Adherent

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease Normal

Age Neonatal

Gender Male

Ethnicity Batch-specific

Applications

Response to pathogens, skin aging, wound healing, gene delivery, skin diseases (e.g., scleroderma)

Product Format frozen 1 mL

Storage Conditions -130°C or below

Comments

Serum-free medium supports excellent growth curves and normal morphology, as well as serum-free (not animal-free) experimental conditions. The presence of 2% fetal bovine serum in the Fibroblast Growth Kit-Low serum (ATCC PCS-201-041) supports more prolific growth.

Complete Growth Medium

Obtain one growth kit from the freezer; make sure that the caps of all containers are tight.
Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath, and shake to dissolve any precipitates prior to adding to the basal medium.
Obtain one bottle of Fibroblast Basal Medium (480 mL) from cold storage.
Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
Using aseptic technique and working in a laminar flow hood or biosafety cabinet, transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.
Tightly cap the bottle of complete growth medium and swirl the contents gently to assure a homogeneous solution. Do not shake forcefully to avoid foaming. Label and date the bottle.
Complete growth media should be stored in the dark at 2°C to 8°C (do not freeze). When stored under these conditions, complete growth media is stable for 30 days.

Table 1. If using the Fibroblast Growth Kit–Serum-Free (ATCC® PCS-201-040), add the indicated volume for each component in the order shown.

Component

Subculturing

Volume

Volume

Final Concentration

Sterility Tests

L-glutamine

Viral Testing

18.75 mL

Viability

7.5 mM

Population Doubling Capacity

Hydrocortisone Hemisuccinate

C of A

0.5 mL

序號	文章標題	操作
1	ATCC細胞運輸和處理	查看詳情
2	其他海外品牌代理	查看詳情
3	ATCC代理	查看詳情
4	購買須知	查看詳情
5	訂購流程	查看詳情

梅經(jīng)理	17280875617	1438578920
胡經(jīng)理	13345964880	2438244627
周經(jīng)理	17757487661	1296385441
于經(jīng)理	18067160830	2088210172
沈經(jīng)理	19548299266	2662369050
李經(jīng)理	13626845108	972239479

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