Restriction digests of the clone give the following sizes (kb): EcoRI/XbaI--3.1, 1.4; EcoRI--4.5; XbaI--4.5; PstI--4.5; HincII--4.5. The insert contains the following restriction sites (nt position according to the published sequence): NdeI--2220; XmnI--2410. Contains the 5'XbaI/3'EcoRI fragment corresponding to nt 1599 to 2978 of the published sequence. The largest ORF extends from nt 88 to 2574. |
