Restriction digests of the clone give the following sizes (kb): BamHI--4.9, 0.7; HindIII--4.4, 1.4; KpnI--5.0, 0.64; PvuII--2.5, 1.7, 1.5; SalI--3.0, 2.7. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--2.08; BglI--0.11; EcoRI--0.97, 1.05; HindIII--1.38; KpnI--0.60; PstI--2.17, 2.30; PvuII--1.16; SacI--1.30; XhoI--2.20, 2.31. The SalI insert contains the complete coding sequence and a poly(A) tail followed by a cloning artifact of approximately 180 bp. The genomic sequence is available in GenBank/EMBL/DDBJ accession J04988. |
