Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Preceptrol®
no
Genotype
F- ara-14 recB21 recC22 sbcB15 lambda-
Type Strain
no
Comments
Bacteriophage host
A host strain for growth of lambda clones containing DNA with substantial secondary structure
Constructed by A.R. Wyman.
The phenotype of the strain originally deposited was F- ara-14 leu-6 his-4 recB21 recC22 sbcB15 lambda-. When the strain was examined in July 1996, it had lost the leu- his- phenotype.
Medium
ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Name of Depositor
D Botstein
References
Wyman AR, et al. Propagation of some human DNA sequences in bacteriophage lambda vectors requires mutant Escherichia coli hosts. Proc. Natl. Acad. Sci. USA 82: 2880-2884, 1985. PubMed: 3157994
Cosloy SD, Oishi M. Genetic transformation in Escherichia coli K12. Proc. Natl. Acad. Sci. USA 70: 84-87, 1973. PubMed: 4630612
