| Vector Information |
Size (kb): 9.2370004653930660 Vector: pMMB277 (plasmid) Promoters: Promoter tac Construction: pMMB206, pMMB311 Marker(s):cmlR Construct size (kb): 9.237000465393066 Features: insert detection: lacZ'
marker(s): cmlR
promoter: tac
replicon: oriV, oriT (IncQ)
repressor gene: lacIq
transcription terminator: rrnB |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; EcoRV--4.2, 1.7, 1.5, 1.1, 0.9; EcoRI/MluI--8.4, 0.8. Contains sequences encoding lacIq, a tac promoter, and lacZalpha to allow direct screening by inactivation of alpha-complementation. Constructed by replacing the MluI/EcoRI fragment of pMMB206 (taclacUV5 promoter) with the MluI/EcoRI fragment from pMMB311 (tac promoter). |
