Restriction digests of the clone give the following sizes (kb): EcoRI/KpnI--2.9, 0.40; PstI--3.2; SstI--3.2, 0.2; BamHI--3.0, 0.3; HindIII--3.2. The insert contains the following restriction sites (approximate kb from the 5' end): SstI--0.05; BamHI--0.26. A PCR product derived from exon 2 and corresponding to nt 27-385 of the sequence record. Vector name: pBluescript KS M13(+). Transcription from the T7 promoter gives the sense strand. |
