Restriction digests of the clone give the following sizes (kb): PstI--7.2, 0.47; EcoRI--8.0; EcoRI/HindIII--7.2, 0.49; BamHI--8.0; AvaI--7.2, 0.4. This sequence is derived from a region upstream of the leader segment of a Vkappa pseudogene of subgroup III. The 10.5 kb constant fragment is derived from a cross-hybridizing sequence, perhaps on chromosome 2 or 22. |
