| Comments |
When excising insert by EcoRI/BglI, the vector is cut in 2, and the insert, which is the largest band, has about 150 bp of pUC18. Restriction digests of the clone give the following sizes (kb): BglI--4.1, 1.15; BamHI--5.2; EcoRI--5.2; SalI--5.2; EcoRI/BglI--2.85, 1.35, 1.05; EcoRI/HindIII--2.7, 2.7. Enzyme(s) not detecting polymorphism: MspI, TaqI, EcoRI, BamHI (in a limited study). |
