| Vector |
Vector: pBluescript SK- (phagemid)
Size (kb): 2.960
Construction: pUC19
Marker(s): ampR (50 ug/ml)
Features: Insert detection: lacZ'; Promoters: T3, T7, lac; Replicons: f1, pMB1; MCS: KpnI...SacI
Sequencing primers: M13(-21), M13 reverse, T7, T3 |
| Insert |
DNA: cDNA
Insert lengths(kb): 3.25
Tissue: cystic fibrosis sweat gland
Gene product: cystic fibrosis transmembrane conductance regulator [CF]
Alleles: A1, A1, A1, A1, A2, B1, B2, A1, A1, A2, B1, B2, A2, A2, A2, A2 |
| Comments |
Contains 2 internal EcoRI sites. An EcoRI digest of the plasmid results in fragments of 1.0, 1.5, and 0.75 kb from the insert. Restriction digests of the clone give the following sizes (kb): EcoRI--2.65, 1.5, 1.1, 0.89; XbaI--6.2; PstI--6.2; BglII--5.7, 0.45; HindIII--3.2, 1.85, 0.94. ATCC 61161 has been converted to meet the requirements of the Budapest Treaty for patent deposits. A compliance form is not needed for ATCC 61161, but is for ATCC 61160. This probe contains a 3 bp deletion (deltaF508) in exon 10. This clone includes exons 9 - 24 and hybridizes to genomic EcoRI fragments of 10.3, 4.3, 3.5, 2.4, 0.5, 1.8, 1.8, 3.5, 1.9, 4.5, 2.4, 1.0. 2.8, 1.8, 8.5, 2.2, and 2.3 kb. |
| References |
Riordan JR, et al. Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science 245: 1066-1073, 1989. PubMed: 2475911
J R Riordan, personal communication
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