| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.6, 3.0; BamHI/EcoRI--3.6, 2.7, 0.36; EcoRI--3.9, 2.7; HindIII--3.0, 1.1, 0.98, 0.88, 0.64; PstI--4.4, 1.1, 0.6, 0.47. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.33; PstI--1.82, 2.88, 3.34. This insert includes the 39 bp exon that can be alternatively spliced. The EcoRI fragment contains some non-cDNA sequence, including a NotI site at both ends. |
