| 產品名稱 |
Vaccinia virus ts mutant |
| 商品貨號 |
B228256 |
| Classification |
Poxviridae, Orthopoxvirus |
| Deposited As |
Vaccinia virus, Orthopoxvirus |
| Agent |
Vaccinia virus ts mutant |
| Strain |
IHD-W Dts36 (Dales isolate 6003) |
| Applications |
The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U10. Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. |
| Positive for Mycoplasma |
No |
| Biosafety Level |
2 CDC BMBL recommends vaccination
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
derived from existing strain |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Comments |
Selected after mutagenesis with nitrosoguanidine and replication in the presence of bromodeoxyuridine. The non-permissive incubation temperature is 39.5oC. This mutant was not assigned a Dales EM category. The mutation was assigned by complementation analysis (Lackner, et al, 2003) to vaccinia map location U10. Vaccination recommended for lab staff manipulating vaccinia, monkeypox, or cowpox viruses (CDC/NIH Biosafety in Microbiological and Biomedical Laboratories, 4th ed, 1999 - stock no. 017-040-00547-4). |
| Effect on Host |
CPE: enlargement, plaques with rounding and eventual sloughing |
| Recommended Host |
Recommended Host: BSC-40 (ATCC CRL-2761 |
| Growth Conditions |
Temperature: 31.0°C Duration: 1-3 days; For best results, cells should be 24 to 48 hours old and 80-90% confluent at time of infection [not 100% confluent]. |
| Name of Depositor |
S Dales, RC Condit, S Dales |
| Source |
derived from existing strain |
| References |
Dales S, et al. Biogenesis of vaccinia: isolation of conditional lethal mutants and electron microscopic characterization of their phenotypically expressed defects. Virology 84: 403-428, 1978. PubMed: 622807
Condit RC, et al. Isolation, characterization, and physical mapping of temperature-sensitive mutants of vaccinia virus. Virology 128: 429-443, 1983. PubMed: 6577746
Lackner CA, et al. Complementation analysis of the Dales collection of vaccinia virus temperature-sensitive mutants. Virology 305: 240-259, 2003. PubMed: 12573570
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