vector permitting visual detection of recombinants beta-galactosidase beta-D-galactosidase
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.4; HindIII--5.4; PstI--5.4.
A low copy-number vector compatible with plasmids having pMB1 or p15A origins of replication. Permits blue/white screening for recombinants.
The copy number is estimated at 10 copies per cell.
Recommendation for verification: BamHI; HindIII; PstI.
Media
ATCC Medium 1065 (see below) plus 30.0 mcg/ml chloramphenicol ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 37.0°C
References
Xu SY, Fomenkov A. Construction of pSC101 derivatives with Camr and Tetr for selection or LacZ for blue/white screening. BioTechniques 17: 57, 1994. PubMed: 7946313
