Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 0.92; HindIII--2.7, 0.94; BamHI--3.5, 0.24; BglII--3.7; PvuII--2.4, 1.25. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.05; BglII--0.23; BamHI--0.80. Nucleotides 1-921 of the insert correspond to nt 2136-3056 of the sequence record. Encodes an internal region of the protein. One of 4 clones (pI7-11, pF5-1, pA2-10, pA2-13, ATCC 65988-65995) that together contain the complete coding sequence. Cloned in the antisense direction. The 3' EcoRI site was derived from the polylinker. |
