Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen
Shipping Information
Shipped: ?Freeze dried Escherichia coli
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
A chloramphenicol resistant host, expressing tnpR resolvase and the cI lambda repressor, for use in the Tn1721-derived site-specific recombination systems and excision of plasmids from the vector lambdaRESII (ATCC 77404).
Contains two copies of the tnpR gene, one under control of the tac promoter for increased inducible expression.
The order of the major features in the transposon is: inverted repeats - EcoRI - tnpR - tac promoter - EcoRI - BamHI - cI - PstI - cmlR - HindIII - res resolution site - tnpR - tnpA - EcoRI - inverted repeats.
Medium
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C
Atmosphere: aerobic
Name of Depositor
J Altenbuchner
References
Altenbuchner J. A new lambda RES vector with a built-in Tn1721-encoded excision system. Gene 123: 63-68, 1993. PubMed: 8380784
