pSP65:TPMV #6 [full-length clone of tomato planta macho viroid]
商品貨號(hào)
B228534
Designations
pSP65:TPMV #6 [full-length clone of tomato planta macho viroid]
Species
Tomato planta macho viroid
Depositors
RA Owens, Beltsville Agricultural Research Center
Applications
Noninfectious unless TPMV cDNA insert is released by BamHI. To use as a template, plasmid must be linearized with HindIII.
Vector
Name of construct: pSP65:TPMV #6
Excise insert: BamHI
Size of construct (kb): 3.36
Markers: ampR
Name of vector: pSP65
Intact vector size: 3.010
Type of vector: plasmid
Vector end: BamHI
Vector end: BamHI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI AvaI SmaI SacI
EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: SP6
replicon: pMB1
terminator: none
Insert
Genome: tomato planta macho viroid
Gene name: genome, full-length
Contains complete coding sequence?: U
Type of DNA: cDNA
Insert end: BamHI
Insert end: BamHI
Insert size (kb): 0.36
Insert Size (kb)
0.36
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
The insert contains an internal SalI site. EcoRI + SalI or HindIII + SalI are diagnostic for the insert orientation.
Restriction digests of the clone give the following sizes (kb): BamHI--3.1, 0.36; HindIII--3.4; EcoRI--3.4.
Noninfectious unless TPMV cDNA insert is released by BamHI. To use as a template, plasmid must be linearized with HindIII.
Full-length (360 bp) TPMV cDNA with BamHI ends was cloned into BamHI of pSP65. Constructed by R.W. Hammond.
Classification
Pospiviroid
References
Kiefer MC, et al. Structural similarities between viroids and transposable genetic elements. Proc. Natl. Acad. Sci. USA 80: 6234-6238, 1983. PubMed: 6312450
