vector containing primer sites useful for sequencing
vector for constructing cDNA libraries
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.7; HindIII--2.7; EcoRI--2.7; XhoI--2.7.
Expression vector useful for construction of cDNA libraries and containing priming sites useful for sequencing. Permits visual detection of recombinants by beta-galactosidase alpha peptide complementation.
pOM1 (ATCC 77220) and pOM2 (ATCC 77221) differ in the orientation of the polylinker.
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References
Oberbaumer I. New pUC-derived expression vectors for rapid construction of cDNA libraries. Gene 49: 81-91, 1986. PubMed: 2436975
Oberbaumer I. Blue libraries are beautiful. Focus 14: 72, 1992.
