Restriction digests of the clone give the following sizes (kb): EcoRI--4.2, 0.74; PvuII--4.9; EcoRI/PvuII--2.4, 1.5, 0.79; BamHI--uncut; HindIII--uncut; AccI--3.3, 1.7. This PstI RFLP is in strong linkage disequilibrium (87% of maximal) with the 5' BglI RFLP detected at this locus by pSPT-PGK. Insert contains the last 83 bp of exon 4 and extends 820 bp into the fourth intron of human PGK1. Occasionally a faint 5.2 kb autosomal band is detected with the PstI RFLP. The smaller EcoRI fragment has been used as the probe. Based on the restriction data and the tetracycline sensitivity of this construct, the vector must not be the reported pBR322. |
