Restriction digests of the clone give the following sizes (kb): BamHI--4.5; EcoRI--3.7, 0.25 (doublet), 0.08 (doublet); PstI--4.5; SalI--4.5; HindIII--3.0, 1.4. The insert contains the following restriction sites (nt from the 5' end): EcoRI--67, 319, 571, 655. Detects human genomic restriction fragments of the following sizes (kb): EcoRI--5.3; HindIII--1.4. Corresponds to nt 94-1469 of the published sequence, and contains coding sequences for the zinc finger domain. Subcloned from lambdaZNF41-3. Transcription from the T7 promoter gives the sense strand. |
