Restriction digests of the clone give the following sizes (kb): SacI--3.1, 1.2; AvaI--3.5, 0.65, 0.3; EcoRI--3.1, 1.2; BamHI--4.3; HindIII--4.3; XbaI--4.3. The insert contains the following restriction sites [nt from the 5' end (position according to the published sequence)]: SacI--28 (140); PvuII--123 (235), 459 (571), 617 (729); 802 (914); PstI--223 (335), 286 (398); KpnI--679 (791). Contains the complete coding region (nt 205 - 1020 of the published sequence). This insert begins at nt 113 of that sequence. Detects a mRNA of 1.4 kb in Hep G2 cells. |
