Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 0.95; HindIII--4.2; BamHI/KpnI--2.2, 2.0. Compatible with broad-host-range plasmids of the incompatibility groups P1, P4/Q and W, and maintained at a copy number of 15-20 in P. savastanoi. Contains the basic replicon of pPS10 which has been sequenced (EMBL accession number X58896). The repA portion is flanked by EcoRI sites (nt 536 and 1500), with the site at nt 536 between the repA promoter and coding sequences. General purpose vector. Derived from the ligation product of a 7.6 kb PstI fragment of pPS10 and a 4.2 kb PstI fragment of pKT231 which was then shortened by deletion. A 1.8 kb region of pPS10 was retained (EMBL accession number X58896) which encodes replication functions. The order of the major features in this plasmid is: PvuII - BamHI - kanR - HindIII - KpnI - repA. |
