For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit). The insert contains nt 485-1516 of Genbank X03453. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.36; PvuI--0.46; ClaI--0.81. The following restriction enzymes are recommend for linearizing the plasmid for in vitro transcription: T3 = SmaI; T7 = XhoI. Restriction digests of the clone give the following sizes (kb): SmaI--4.0; SmaI/XhoI--3.0, 1.1; XhoI--4.0. |
