Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 1.6; AccI--3.3, 0.86, 0.3; XhoI--3.4, 0.76, 0.46; BamHI--4.6; PvuII--2.4, 1.5, 0.64. The insert contains the following restriction sites (approximate kb from the 5' cloning site): EcoRI--0.03, 0.04, 0.12; AccI--0.4, 0.64, 0.9; BstXI--0.41; XhoI--0.6, 1.28. Detects human genomic restriction fragments of the following sizes (approximate kb): BamHI--23; EcoRI--10; HindIII--15; Contains the complete coding sequence, with 5' and 3' untranslated regions. and an mRNA in HeLa cells of 1.7 kb. There are about 40 bp between the 5' reported sequence and the vector cloning site (adjacent to the T3 promoter). This region contains 3 EcoRI sites. |
