Restriction digests of the clone give the following sizes (kb): EcoRI--2.9, 1.15; HindIII--3.0, 1.0; BamHI--3.7, 0.25; PstI--2.95, 1.05; KpnI--4.0. The insert contains the following restriction sites (approximate kb from the 5' end): HindIII--0.2, 0.78; BamHI--0.88. Contains part of intron 1 and extends to an EcoRI site in intron 2. Encodes part of the first leader and the first immunoglobulin variable region-like domain. Vector name: pBluescript KS M13(+). Transcription from the T3 promoter gives the sense strand. |
