Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Protocol: ATCCNO: 50521 SPEC: Monoprotist culture, cultivated with mixed bacteria. Place thawed contents of ampule into T-25 flask containing 10 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC-13048 as a food source. Subculture every 4-8 weeks (when maintained at 25C; cysts are stable longer at lower temperatures) as follows: Rub the surface of the agar with a sterile swab, agitate vigorously and aseptically transfer a 0.1 ml aliquot to a fresh flask of bacterized medium.
Subcultivation
Protocol: ATCCNO: 50521 SPEC: Monoprotist culture, cultivated with mixed bacteria. Place thawed contents of ampule into T-25 flask containing 10 ml of ATCC medium 802 bacterized with Enterobacter aerogenes ATCC-13048 as a food source. Subculture every 4-8 weeks (when maintained at 25C; cysts are stable longer at lower temperatures) as follows: Rub the surface of the agar with a sterile swab, agitate vigorously and aseptically transfer a 0.1 ml aliquot to a fresh flask of bacterized medium.
