To convert bacteriophage lambda clones to plasmid via the cre-lox recombination system, the use of host strain E. coli BNN 132 (ATCC 47059) is recommended.
Vector
Tissue: B lymphocyte (EBV transformed)
DNA: cDNA
Insert size range: 0.7 - 7 kb
Insert size range (kb): 0.7 - 7
Insert ends: XhoI adaptors
Insert site: XhoI
Number of independent recombinants: 1 x 10^8
Non-recombinants (%): 5
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
To convert bacteriophage lambda clones to plasmid via the cre-lox recombination system, the use of host strain E. coli BNN 132 (ATCC 47059) is recommended.
References
Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010
