Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Allows the recovery of bacteriophage with recombinant DNA containing secondary structure, but supports poorer phage growth than PMC103 and PMC128 (ATCC® 47060™, 47064™).
This strain is being made readily available to the scientific community with the proviso that it not be used to form the basis of a product for commercial sale or be included in any commercial product (personal communication).
Constructed from JC12186 by P1 transduction of delta(mcrBC-hsdRSM-mrr) from Escherichia coli ER1648.
Methylation tolerant, recombination deficient host that improves the stability of cosmid clones
Medium
ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor
JP Doherty
References
Doherty JP, et al. Escherichia coli host strains SURE (TM) and SRB fail to preserve a palindrome cloned in lambda phage: improved alternate host strains. Gene 124: 29-35, 1993. PubMed: 8440479
