| 產品名稱 |
HFH 15-9 |
| 商品貨號 |
B230237 |
| Designations |
HFH 15-9 |
| GenBank Number |
U16861
|
| Species |
Homo sapiens, human |
| Depositors |
L Wood |
| Vector |
Construct size (kb): 45.29999923706055 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt11
Intact vector size: 43.700
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.2 kb
Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
promoter: lac
replicon: lambda
repressor gene: cI857
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: KCNJ2
Other gene symbols: IRK1
Gene name: potassium inwardly-rectifying channel, subfamily J, member
2
Contains complete coding sequence?: Y
Tissue: fetal heart
Type of DNA: cDNA
Insert end: Modification: EcoRI linker
Insert end: Modification: EcoRI linker
Insert size (kb): 1.671
Cross references: DNA Seq. Acc.: U16861
Nucleotides 1-1671 of the insert correspond to
nucleotides 1-1671 of U16861. Insert lengths(kb): 1.671000003814697 Tissue: fetal heart Gene product: potassium inwardly-rectifying channel, subfamily J, member 2 [IRK1] |
| Insert Size (kb) |
1.671 |
| Media |
ATCC® Medium 1592: SM buffer
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: Frozen |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI-26.0, 20.0; HindIII--25.0, 21.0, 9.5, 5.6; XbaI--25.0, 22.0, 19.0. The insert contains the following restriction sites (approximate kb from the 5' end): KpnI--0.53; HindIII--0.69; ClaI--0.84, 1.10; XbaI--1.57. |
| References |
Wood LS, et al. Cloning and functional expression of a human gene, hIRK1, encoding the heart inward rectifier K+-channel. Gene 163: 313-317, 1995. PubMed: 7590287
|
