Insert contains the following restriction sites (with positions according to the published sequence): PstI--678, 1581. Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 1.9; PstI--3.7, 0.9; HindIII--4.6. The probe detects bovine cerebellar mRNA of 3.1 and 2.4 kb and rat brain mRNA of 3.1 kb. Used to map the human gene. It detects human genomic HindIII fragments of 5.5, 4.6, and 4.3 kb and EcoRI fragments of 7.2, 4.4, and 3.4 kb. A 170 bp HincII/EcoRI fragment of the insert detects a major genomic bovine PvuII fragment of 10.5 kb and weaker signals at 5.7 and 7.6 kb. In humans this fragment detects a genomic 7.2 kb PvuII fragment. |
