Restriction digests of the clone give the following sizes (kb): BamHI--4.4; HindIII--4.4; PvuII--4.4. Expression can be induced in cells grown in low osmolarity media by the addition of sodium chloride. Vector does not provide a ribosome binding site or initiation codon. Expression vector allowing osmotically controlled expression of cloned inserts directed by the E. coli proU promoter. This material is being provided with the explicit understanding that it not be used for commercial purposes without prior authorization from the depositor. Vector constructed from pOSEX2 (ATCC 87211) by deletion of a portion of the pBR322 backbone. |
