| Vector |
Construct size (kb): 5.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lafmid BA
Intact vector size: 3.153
Type of vector: phagemid
Vector end: HindIII
Vector end: NotI
Cloning sites: HindIII, BamHI, NotI, EcoRI
Polylinker sites: HindIII, BamHI, NotI, EcoRI
Other unique sites: ClaI, XmnI, ScaI, ApaLI
Construction: pGEM
Host range: Escherichia coli
Features (with orientation and position when available):
replicon: f1, <-, 100-556
insert detection: lacZ', <-, 594-1119
primer site: M13 universal, ->
MCS: EcoRI/NotI/BamHI/HindIII, ->, 1061-1098
primer site: M13 reverse, <-
promoter: lac, <-, 1164-1192
replicon: pMB1, <-, 1518-1518
marker(s): ampR, <-, 2276-3136
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: TEGT
Genomic copy number: unique
Gene name: testis enhanced gene transcript
Contains complete coding sequence?: N
Chromosome: 12
Tissue: infant brain, 3 months old (from Bento Soares)
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 2.5
Cross references: DNA Seq. Acc.: T08698; EST: EST06590; Primer: M13-21; THC
id: THC123576
DNA Seq. Acc.: T08699; EST: EST06591; Primer: M13 Reverse;
THC id: THC123576
DNA Seq. Acc.: X75861; EST: EST06591
Nucleotides 1-363 of the insert correspond to
nucleotides 58-420 of X75861.
DNA Seq. Acc.: X75861; EST: EST06590
Nucleotides 2174-2509 of the insert correspond to
nucleotides 2231-2566 of X75861 with 99% identity. Insert lengths(kb): 2.5 Tissue: infant brain, 3 months old (from Bento Soares) Gene product: testis enhanced gene transcript(TEGT) [TEGT] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; HindIII/NotI--3.3, 1.75, 0.8. Corresponds to nt 58-2566 of GenBank X75861, and contains most of the coding sequence (nt 41-754). Verified to contain EST06590 by single-pass sequencing. Related product(s): oligonucleotides, ATCC 73233. |
