| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--9.8; PvuII--4.4, 3.6, 1.7; EcoRI--7.1, 2.7; BamHI--9.8, HindIII--5.4, 4.4. Digestion with BglII and HindIII together release 4 DNA fragments of 2.5, 1.8, 0.6, and 0.5 kb. The 0.6 and 0.5 kb fragments are unique in the genome and can be used as a hybridization probe to detect a 5 kb mRNA (3' end). |
| References |
Royer-Pokora B, et al. Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location. Nature 322: 32-38, 1986. PubMed: 2425263
Monaco AP, et al. Detection of deletions spanning the Duchenne muscular dystrophy locus using a tightly linked DNA segment. Nature 316: 842-845, 1985. PubMed: 2993910
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