This is a non-pathogenic E. coli strain that is recommended by the USDA as a surrogate indicator organism to measure changes in microbial counts.
Isolation
Cattle Hides
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
freeze-dried
Storage Conditions
Frozen: -80°C or colder Freeze-Dried: 2°C to 8°C Live Culture: See Propagation Section
Preceptrol®
no
Type Strain
no
Comments
Non-toxigenic isolate.
The depositor states that no cytotoxicity was observed with African green monkey kidney (Vero) cells.
The lack of virulence factor genes has been confirmed by the E. Coli Reference Center of Pennsylvania State University (see virulence gene profile).
Medium
ATCC® Medium 18: Trypticase Soy Agar/Broth
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Name of Depositor
JS Dickson, K Marshall
Special Collection
ATCC
References
Marshall K M, et al. Identification of Escherichia coli O157:H7 Meat processing Indicators for Fresh Meat through Comparison of the Effects of Selected Antimicrobial Interventions. J. Food Prot. 68: 2580-2586, 2005.
