Restriction digests of the clone give the following sizes (kb): PstI--2.7; BamHI--2.7; HindIII--2.7; EcoRI--2.7. Expression vector permitting production of a fusion protein and visual detection of recombinants, using the beta-galactosidase alpha peptide as the reporter group. Distributed in aliquots of 2 ug (100 ng/ul). A pUC19 derivative with 2 XmaI sites--one at nt 412 in the multiple cloning site and one added at nt 1567 by replacing DNA between the DraI sites at nt 1563 and 1582 with phosphorylated d[pCCCCGGGG]. |
