Restriction digests of the clone give the following sizes (kb): EcoRI--3.9; BglII--3.3, 0.54; HindIII--2.6, 0.88, 0.33; PstI--3.2, 0.64; XbaI--3.4, 0.43; HindIII/SstI--2.7, 0.76, 0.34. The insert contains the following restriction sites (approximate kb from the 5' end): BglII--0.55; HindIII--0.30; NcoI--0.46; PstI--0.60, 0.77; StuI--0.76; XbaI--0.41. Overproduces the fusion protein in E. coli, the majority of which is found in inclusion bodies. It binds estrogens and antiestrogens. Contains coding sequence for amino acids 241-595 including the stop codon. The recombinant fusion protein contains an additional 18 N-terminal amino acids encoded by the vector polylinker. |
