| Insert |
DNA: genomic Insert lengths(kb): 20.0 Tissue: liver Gene product: DNA Segment, single copy [D3S14] Alleles: A1, B1, B2, A2, B3 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--22.0 (doublet), 11.5; BamHI--22.0, 11.5, 8.8, 6.0, 4.0; PstI--15.0, 5.8, 3.1, 2.7, 2.2, 1.8, 1.5, 1.2, 0.95; HindIII-->23.0, 6.0, 5.8; BglII--23.0, 9.4, 5.2, 3.4, 2.9, 1.9. The BglII and MspI polymorphisms have a combined PIC value of 0.71 and a combined heterozygosity of 76%. Enzyme(s) not detecting polymorphism: BamHI, EcoRI, HindIII, HincII, PstI, RsaI, TaqI (panel of 5 individuals). |
| References |
Schumm JW, et al. Identification of more than 500 RFLPs by screening random genomic clones. Am. J. Hum. Genet. 42: 143-159, 1988. PubMed: 2892396
Donis-Keller H, et al. A genetic linkage map of the huma genome. Cell 51: 319-337, 1987. PubMed: 3664638
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