| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--7.8, 2.7; PstI--4.2, 3.8, 1.0, 0.65, 0.5, 0.3; EcoRI--5.3, 5.2; HindIII--4.9, 2.6, 2.4, 0.6; SalI--10.5. The insert contains the following restriction sites (nt from the 5' end): PstI--1170 (exon 1), 1603 (intron 1), 5820 (exon 10), 6892, 7523 (exon 12); HindIII--2313 (intron 1), 4935 (exon 8), 7206 (exon 12); EcoRI--5207 (intron 8). Contains the complete gene (12 exons) with 5' and 3' flanking sequences. When the insert is cloned into pSV2neo and transfected into enzyme-deficient CHO cells, the enzyme is synthesized and is inducible by insulin or tetradecanoyl phorbol acetate. |
