| 產品名稱 |
pSV2-gpt |
| 商品貨號 |
B231907 |
| Designations |
pSV2-gpt |
| Depositors |
P Berg |
| Biosafety Level |
1 |
| Host |
Escherichia coli HB101 |
| Vector Information |
Size (kb): 5.50
Vector: pSV2-gpt (plasmid)
Promoters: Promoter SV40
Construction: SV40, pBR322, Escherichia coli
Marker(s): ampR,gpt+
Construct size (kb): 5.5
Features:
marker(s): ampR, gpt+
promoter: SV40
replicon: pMB1
terminator: SV40 early
|
| Applications |
Expression vector
Shuttle vector |
| Comments |
Restriction digests of the clone give the following sizes (kb): HindIII/BglII--5.2, 0.12; HindIII--5.2; HindIII/EcoRV--4.7, 0.58; PvuII--5.3; PstI--4.3, 0.9; PvuII/PstI--2.8, 1.5, 0.9. Plasmid containing cells use xanthine as a guanine nucleotide precursor. |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Growth Conditions |
Temperature: 37°C |
| References |
Mulligan RC, Berg P. Expression of a bacterial gene in mammalian cells. Science 209: 1422-1427, 1980. PubMed: 6251549
Mulligan RC, Berg P. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Proc. Natl. Acad. Sci. USA 78: 2072-2076, 1981. PubMed: 7017722
|
| Shipped |
frozen |
