| Vector Information |
Size (kb): 5.6030001640319820 DESCRIPTION OF VECTOR:
Intact vector size: 5.603
Type of vector: plasmid
Cloning sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Polylinker sites: EcoRI SacI KpnI SmaI BamHI XbaI HincII AccI SalI PstI SphI
HindIII
Other unique sites: EcoRV NdeI AatII NarI SpeI NaeI NheI BglII ClaI NsiI StuI
Construction: pUC19
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
promoter: lac, ->, 143-171
insert detection: lacZ', ->, 216-500
MCS: HindIII...EcoRI, ->, 234-285
centromere: CEN4, 504-1480
replicon: ARS1, 1481-2225
marker(s): URA3, <-, 2563-3365
marker(s): ampR, ->, 3802-4662
replicon: pMB1, 5420-5420 Vector: YCplac33 (plasmid) Promoters: Promoter lac Construction: pUC19 Marker(s):URA3,ampR Construct size (kb): 5.603000164031982 Features: insert detection: lacZ'
marker(s): URA3
marker(s): ampR
promoter: lac
replicon: ARS1
replicon: pMB1
MCS: HindIII...EcoRI
centromere: CEN4 |
| Comments |
Restriction digests of the clone give the following sizes (kb): AvaI--3.7, 1.9; ClaI--5.7; PstI--5.6. One of 9 shuttle vectors (ATCC 87585 - 87593) allowing lacZ detection of cloned inserts and containing the pUC19 MCS with all 10 cloning sites unique. Vectors differ in mode of replication (YE, YC or YI-type) and yeast marker (LEU2, URA3 or TRP1). The PstI restriction site from the URA3 gene of S. cerevisiae were removed by using oligo-directed mutagenesis. |
