| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; PstI--2.7; BglI--2.7; HindIII--2.7; BamHI--2.7. The following unique restriction sites are found on this vector separated by (bp)(approx): BglI- 100- PstI- 150- PvuI- 1020- EcoRI- 10- BamHI-HindIII-1420. This vector is used for expression of fragments containing a ribosome binding site. The orientation of the PL promoter is clockwise with respect to the plasmid ori. |
| References |
Remaut E, et al. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene 15: 81-93, 1981. PubMed: 6271633
Anderson DM, et al. DNA sequences encoding bovine interleukin-2. US Patent 4,882,282 dated Nov 21 1989
Namen AE, et al. Interleukin-7. US Patent 4,965,195 dated Oct 23 1990
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