Restriction digests of the clone give the following sizes (kb): BglII/XbaI--3.0, 1.4; PstI--3.9, 0.5; KpnI--4.4. Insert contains the following restriction sites (approximate kb from the 5' end): NdeI--0.46; PstI--0.98; PvuII--1.38; SacI--0.29; StuI--1.05. Insert lacks the coding sequence for the N-terminal 29 amino acids. Nuclotides 1 - 1415 of the insert match nt 212 - 1627 of GenBank AF002999 with 98% identity and 17 gaps. The first nucleotide after the BglII site is the start of the insert. |
