Restriction digests of the clone give the following sizes (kb): EcoRI--4.4; HindIII--4.4; EcoRI/HindIII--3.0, 1.45; BglI--4.4; PvuII--4.4. Insert can be labeled by transcription with SP6 polymerase on HindIII linearized plasmid. Codominant segregation shown in 2 informative families (10 individuals). The locus D21S82 is very closely linked to the locus SOD1 on chromosome 21. No background at stringency of 0.2X SSC, 65C. Enzyme(s) not detecting polymorphism: BglII, HindIII, MspI, PstI, RsaI and TaqI, in 5 unrelated Caucasians. |
