| Vector |
Construct size (kb): 5.800000190734863 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pUC13
Intact vector size: 2.700
Type of vector: plasmid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: HindIII PstI SalI AccI HincII XbaI BamHI SmaI SacI EcoRI
Polylinker sites: HindIII PstI SalI AccI HincII XbaI BamHI SmaI SacI EcoRI
Construction: pBR322, Escherichia coli lacZalpha, M13mp11
Host range: Escherichia coli
Features (with orientation and position when available):
enhancer: none
insert detection: lacZ'
marker(s): ampR
promoter: lac
replicon: pMB1
terminator: none
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: ENG
Other gene symbols: HHT1; ORW1; END
Gene name: endoglin (Osler-Rendu-Weber syndrome 1)
Contains complete coding sequence?: Y
Chromosome: 9; Localization: 9 q34.1
Tissue: HL-60 cell line, PMA-treated
Type of DNA: cDNA
Insert end:
Insert end:
Insert size (kb): 3.1
Cross references: DNA Seq. Acc.: X72012
Nucleotides 1-3073 of the insert correspond to
nucleotides 1-3073 of X72012. Insert lengths(kb): 3.099999904632568 Tissue: HL-60 cell line, PMA-treated Gene product: endoglin (Osler-Rendu-Weber syndrome 1)(Endoglin) [END] |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.2, 2.7; HindIII--5.9; BamHI/MluI--3.0, 2.3, 0.58; BamHI--3.0, 2.9; PstI--4.4, 0.87, 0.68. The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--2.7; BglI--3.0; PstI--0.33, 1.04, 1.28. Encodes the S-isoform with a monomeric molecular weight of 85 kDa. This form, which is not the predominant one, includes an exon of 135 bp. |
