| 產品名稱 |
PEmbl 600 [pEmbl 600 q] |
| 商品貨號 |
B233164 |
| Designations |
PEmbl 600 [pEmbl 600 q] |
| Species |
Homo sapiens, human |
| Depositors |
R Padua |
| Applications |
DNA from secondary transfectants was used to isolate the clone. |
| Vector |
Construct size (kb): 4.400000095367432 |
| Insert |
DNA: genomic Insert lengths(kb): 0.6000000238418579 Tissue: NK14 cell line DNA transferred into NIH 3T3 cells Gene product: mel transforming oncogene (derived from cell line NK14)- RAB8 homologue(ras-associated protein RAB8) [MEL] |
| Insert Size (kb) |
0.600 |
| Media |
ATCC® Medium 1065: LB Agar/Broth, Miller
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): AvaI--3.85, 0.69; ClaI--4.4; EcoRI--4.4; SalI--4.4; HindIII--4.4. Contains Sau3A (225), AccI (300), HinfI (345), DdeI (365), and SacII (540) sites (approximate distances in bp from 5' end). Suggest using the AvaI/Sau3A fragment as there are 50 bp of Alu sequences 3' to Sau3A site. NIH 3T3 cells were transformed with DNA from the melanoma NK14 cell line. DNA from secondary transfectants was used to isolate the clone. |
| References |
Padua RA, et al. A novel transforming gene in a human malignant melanoma cell line. Nature 311: 671-673, 1984. PubMed: 6090953
Spurr NK, et al. Chromosomal assignment of c-mel, a human transforming oncogene, to chromosome 19 (p13.2-q13.2). Somatic Cell Mol. Genet. 12: 637-640, 1986. PubMed: 3466361
Rose. Ann Padua, personal communication
|
